This dataset holds Level 1B (L1B) calibrated radiance data collected by the Portable Remote Imaging SpectroMeter (PRISM) instrument. The data were acquired in October-November 2023 for the Biodiversity Survey of the Cape (BioSCape), an international collaboration between the National Aeronautics and Space Administration (NASA) and the South African National Space Agency (SANSA) to study biodiversity in South Africa's Greater Cape Floristic Region (GCFR). PRISM is a compact image spectrometer that acquires data in the near ultraviolet (UV) to near infrared (IR) spectral bands between 350 and 1050 nanometers with 3.5 nm spectral resolution. PRISM also has a two-channel spot radiometer at short-wave infrared (SWIR) band (1240 nm and 1640 nm) in order to provide accurate atmospheric correction of the ocean color measurements. PRISM's capabilities make it well suited for studies of aquatic and coastal ecosystems. This dataset includes calibrated radiance and observational parameters in netCDF format, quicklook images as TIFF images, and processing information in text-based YAML format.